In situ bioremediation of groundwater has become one of the most widely used technologies for contaminated site treatment because of its relatively low cost, adaptability to site specific conditions, and efficacy when properly implemented. Applicable for both warm and cold temperatureclimate site applications. In situ and ex situ are two methods used to describe different biological processes such as. The detailed results of ex situ controlled aerobic bioremediation of ten sites with differing petroleum contamination and four in situ cases are reported and compared. The grant was this report addresses the results of the ex situ demonstration at funded by the ontario ministry of environment and energys the novartis site. Often a synergistic remedial effect can be attained by using a combination of methods, such as ex situ methods to address grossly contaminated source soils or groundwater. Bioremediation relies on microbes that live naturally in soil and groundwater. Ex situ bioremediation technology includes most of disadvantages and limitations. Pdf on sep 14, 2014, s raja and others published exsitu and insitu bioremediation strategies with special reference to marine and coastal. Ex situ techniques can be faster, easier to control, and used to treat a wider range of contaminants and soil types than in situ techniques. Bioremediation is a process that uses microorganisms or their enzymes to remove pollutants from the environment. The optimization and control of microbial transformations of organic contaminants require the integration of many scientific and engineering disciplines. Mahdi 1biology department, college of science, university of basrah, basrah, iraq.
Bioremediation processes are cost effective, safe and naturebased methods over the chemical and physical methods. In situ and ex situ characterization of the microstructure. Dec 29, 2011 types of in situ bioremediation1 intrinsic in situ bioremediation. The design process commonly includes bench and pilotscale treatability studies. Some examples of bioremediation related technologies are phytoremediation, bioventing, bioleaching, landfarming, bioreactor, composting, bioaugmentation, rhizofiltration, and.
When consider bioremediation, it becomes an ex situ process when the pollutants are collected from their natural location for the treatment. Sep 16, 2016 ex situ bioremediation techniques are unlikely to be used in some sites such as under buildings, inner city and working sites philp and atlas 2005. On the other hand, the excavation features of ex situ bioremediation tend to disrupt soil structure. Bioremediation approaches are generally classified as in situ or ex situ. Ex situ bioremediation environmental biotechnology. Coupling bioflocculation of dehalococcoides to highdechlorination rates for ex situ and in situ bioremediation abstract bioremediation of trichloroethene tce using dehalococcoides mccartyicontaining microbial cultures is a recognized and successful remediation technology. There is more certainty about the uniformity of treatment because of the ability to homogenize, screen, and continuously mix the soil. The soil can, however, be used for landscaping purposes. At these temperatures the soil melts and forms a glass block. Sections 5, 7, 8, 9, and 17 of this policy do not apply to pilot scale facilities. Ex situ bioremediation list of high impact articles ppts. Bioremediation techniques are designed to break down contaminants via the stimulation of microorganisms which use the contamination as an energy source for development and growth. However, they require excavation and treatment of the contaminated soil before and, sometimes, after the actual bioremediation step. Dec 10, 2017 in situ and ex situ bioremediation technologies p2 duration.
Bioremediation experts representing academic research, field practice, regulation, and industry provide accessible information and case examples. Dec 10, 2017 in situ and ex situ bioremediation technologies p1 duration. Ex situ bioremediation of soil contaminated with crude oil by use of actinomycetes consortia for process bioaugmentation 1ahmed a. Ex situ biological treatment one of the main advantages of ex situ bioremediation is that it requires less time than the in situ treatment. In situ and ex situ bioremediation technologies p2 duration. Bioremediation that can occur on its ownfactors effecting rate of intrinsicbioremediation. Ex situ conservation provides less mobility to the organisms due to relatively smaller habitat or area than the in situ. Six pumping wells, two recharge wells and fifteen observation wells were installed for this study as a part of the ex situ biodegradation treatment plant. It has been used commercially for more than 20 years. Ex situ conservation literally means, offsite conservation. In aerobic bioventing, air is typically injected into the subsurface to facilitate aerobic metabolism of hydrocarbons. Various bioremediation techniques can be applied in situ or ex situ and under aerobic or anaerobic conditions.
Groundwater remediation 2 1 introduction groundwater remediation is the cleanup of contaminated groundwater to levels that are in compliance with regulatory limits. Due to the ex situ techniques used to decontaminate the soil, much of the soil cannot be used as filling or agricultural material. In situ bioremediation is the clean up approach which directly involves the contact between microorganisms and the dissolved and sorbed contaminants for biotransformation bouwer and zehnder, 1993. In situ and ex situ application processes to liberate contaminants making them more bioavailable for microbial bacteria degradation. All of these types of bioremediation are receiving increasing attention as viable remediation alternatives for several reasons. Bioremediation techniquesclassification based on site of. In situ bioremediation is the application of biological treatment to the cleanup of hazardous chemicals present in the subsurface. Engineering issue in situ and ex situ biodegradation. The first commercial in situ bioremediation system was installed in 1972 to. Pdf ex situ bioremediation of soil contaminated with crude. Pdf ex situ bioremediation of soil contaminated with.
Gk, general studies, optional notes for upsc, ias, banking, civil services. Sep 17, 2018 the main difference between in situ and ex situ is that the in situ refers to the original location whereas the ex situ refers to the offsite. Technologies grace as part of a grant to complete development of has been applied to both ex situ and in situ treatment of soil. It consists of placing the excavated materials into an above ground enclosure. Ex situ bioremediation of soil ex situ techniques can be faster, easier to control, and used to treat a wider range of contaminants and soil types than in situ techniques. A general essay on bioremediation of contaminated soil. Coupling bioflocculation of dehalococcoides to high. Ex situ bioremediation, in which contaminated soil is excavated and treated elsewhere, is an alternative. The potential effects of sawdust, and mixture of cow and sheep dung to biostimulant autochthonous microflora and augmentation for hydrocarbon bioremediation were investigated in test biopile, made of soil polluted with petroleum waste 100kg. In situ bioremediation an overview sciencedirect topics. Section 2 of the report provides an overview of bioremediation technologies, including in situ and ex situ technologies, and provides examples of field use for three types of bioremediation technologies. Pdf on sep 14, 2014, s raja and others published exsitu and insitu bioremediation strategies with special reference to marine and coastal environments find, read and cite all the research.
In situ and ex situ bioremediation technologies p2 youtube. Upon cooling, organic contaminants are pyrolysed and. Generally, bioremediation technologies can be classified as in situ or ex situ. Bioremediation as a sustainable alternative to chemicalphysical processes is an attractive solution for soil decontamination and renewal of polluted sites. Insitu and exsitu bioremediation technologies p1 youtube. Rather, both in situ and ex situ methods are effective, depending on the facilityspecific characteristics and the nature of the application.
The use of organisms to reduce and transform contaminants into nontoxic substances is an environmentally friendly process which does not ne. The goal in evaluating in situ bioremediation is to assess whether the weight of evidence from tests such as those described above documents a convincing case for successful bioremediation. The main difference between in situ and ex situ is that the in situ refers to the original location whereas the ex situ refers to the offsite. An overview of conventional and innovative technologies. Bioremediation technologies can be generally classified as in situ or ex situ. National parks, sacred forests, lakes, marine parks, biosphere reserves, gene sanctuaries. In ex situ bioremediation, contaminants are treated in some other place from the original site. In situ occurs within the site of contamination 1 in situ bioremediation can further be categorized by the metabolism occurring, aerobic and anaerobic, and by the level of human involvement. Liens p, grotenhuis t, tabak h eds soil and sediment remediation.
These microbes pose no threat to people at the site or in the community. This article discusses the design, operation, performance, and modifications to the remediation system over time. It also suffers from costs associated with solid handling process e. Introduction to in situ bioremediation of groundwater. Some examples of bioremediationrelated technologies are phytoremediation, bioventing, bioleaching, landfarming, bioreactor, composting, bioaugmentation, rhizofiltration, and. Ex situ processes involve the removal of the contaminated media to a treatment area. This technique is usually faster, easier to control and used to treat a wider range soil types and contaminants than in situ techniques.
Fundamental principles of bioremediation an aid to the. This section discusses in situ and ex situ bioremediation. It is concluded, that bioremediation of petroleum contaminated soil is an effective, safe and economically feasible remediation technique for the remediation of such. Ex situ bioremediation can use bioreactors and added nutrients.
Ex situ bioremediation treatment landfarming springerlink. Some examples of bioremediation technologies are bioaugmentation, biostimulation, bioreactors, landbased treatments, and fungal remediation. Combined exsitu and insitu remediation of soil and. It is the process of protecting an endangered species, variety or breed, of plant or animal outside its natural habitat. Biotransformation in the surface environment is a very complex process. The characteristics of bioremediation projects at superfund and other sites are described in section 3. Ex situ bioremediation ex situ bioremediation involves removal of waste materials and their collection at a place to facilitate microbial degradation. Later, the air stripper and carbon units were eliminated, and an infiltration gallery was installed to receive treated, oxygenated effluent in order to promote flushing of the smear zone and in situ bioremediation in the source area. Emily majcher, duane graves, geosyntec consultants. The most feasible bioremediation technologies of unsaturated soils contaminated by petroleum are 1 the onsite controlled remediation of the excavated contaminated plume in the contained system. Mar 03, 2017 key difference in situ vs ex situ bioremediation bioremediation is a term used in biotechnology to refer the process of cleaning the polluted areas using biological organisms such as microorganisms and plants. Ex situ bioremediation involves extraction of contaminated media to other location for treatment.
Increasingly, in situ bioremediation is being heralded as a promising new alternative ground water cleanup technology. Combined exsitu and insitu remediation of soil and groundwater impacts to meet site closure requirements presented by. Ex situ bioremediation processes involve an excavation of contaminated soil or pumping of groundwater before and sometimes, after the actual bioremediation treatment. In situ bioremediation involves treating the contaminated material at the site, while ex situ involves the removal of the contaminated material to be treated elsewhere. Ex situ bioremediation describes a process where contaminated soil or water is removed from the environment by biological organisms. Another advantage is the certainty of the control treatment due to the ability to uniformly screen, homogenize and mix the soil. Difference between in situ and ex situ conservation. This is the key difference between in situ and ex situ bioremediation. Trick to learn in situ on site and ex situ off site conservation duration.
A systematic approach to in situ bioremediation in groundwater, including decision trees on in situ bioremediation for nitrates, carbon tetrachloride, and perchlorate isb8 aug02 provides guidance for the systematic characterization, evaluation, and appropriate design and testing of isb for any biotreatable contaminant. In situ bioremediation isb is the application of bioremediation in the subsurface as compared to ex situ bioremediation, which applies to media readily accessible aboveground e. Pdf on sep 14, 2014, s raja and others published ex situ and in situ bioremediation strategies with special reference to marine and coastal environments find, read and cite all the research. In situ bioremediation involves treating the contaminated material at the site while ex situ involves the removal of the contaminated material to be treated elsewhere. But in situ bioremediation is also clouded in uncertainty, controversy, and mistrust. Ex situ bioremediation techniques are unlikely to be used in some sites such as under buildings, inner city and working sites philp and atlas 2005. Phytoremediation acknowledgements glossary bibliography biographical sketch summary a brief outline of the development of bioremediation technologies is presented.
In situ conservation means the conservation of ecosystems and natural habitats and the maintenance and recovery of viable populations of species in their natural surround. Bioremediation techniques are destruction techniques to stimulate the growth of microorganisms, using the contaminants as a food and energy source. And, ex situ bioremediation means you would scoop up the contaminated soil or pump up the contaminated water and take it somewhere else for bioremediation. Insitu and exsitu bioremediation technologies p1 duration. These tests may be performed during the feasibility study or remedial design, are used to evaluate whether the proposed bioremediation remedy will be successful, and obtain important design criteria. In this the contaminated soil is excavated and removed from the site as completely as possible. In situ bioremediation involves treating the polluted material at the site while ex situ involves the removal of the polluted material to be treated elsewhere aggarwal et al. Fundamental principles of bioremediation an aid to. A major advantage of ex situ bioremediation processes is that most of the decontaminated soil can be reused. Pdf exsitu and insitu bioremediation strategies with. In in situ bioremediation, contaminants are treated at the same site using biological systems.
Investigators cannot fully understand the details of whether and how bioremediation is occurring at a site. Exsitu biological treatment one of the main advantages of exsitu bioremediation is that it requires less time than the insitu treatment. Us epa engineering issue in situ and ex situ biodegradation. Pdf exsitu and insitu bioremediation strategies with special. Furthermore, in situ methods are less expensive and less manageable while the ex situ methods are expensive and manageable. Difference between in situ and ex situ bioremediation. In the use of a bioreactor, contaminated soil is mixed with water and nutrients and the mixture is agitated by a mechanical bioreactor to stimulate. Case study of ex situ remediation and conversion to a. In situ bioremediation environmental biotechnology. Examples of ex situ processes include land treatment and composting. In situ in situ and ex situ ex situ bioremediation technologies for groundwater contaminated with chlorinated solvents michelle m. Ex situ bioremediation occurs at a location separate from the contaminated site and involves the translocation of the contaminated material. Introduction to in situ bioremediation of groundwater es3. Historical information on the development of bioremediation can be found in martin and gershuny, 1992.
Kalogerakis n 2005 ex situ bioremediation of contaminated soils. Ex situ bioremediation of soil contaminated with crude oil by. In situ bioremediation involves treating the contaminated material at the site while ex situ involves the removal of the contaminated material to be. Ex situ bioremediation approaches include use of bioreactors, landfarming, and biopiles. In situ bioremediation or possibly isco to treat residual hydrocarbon in source area during or following free phase recovery. These tests may be performed during the feasibility study or remedial design, are used to evaluate whether the proposed bioremediation remedy will be successful,and obtain important design criteria. Similarities between in situ and ex situ in situ and ex situ are two phenomena of many biological processes classified based on the location where the process is carried out. In situ bioremediation the use of microorganisms for onsite removal of contaminantsis potentially cheaper, faster, and safer than conventional cleanup methods.